Device

Part:BBa_K2018035:Design

Designed by: Joel Vej-Nielsen   Group: iGEM16_SDU-Denmark   (2016-06-24)


phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 981
    Illegal BglII site found at 1806
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 287
    Illegal NgoMIV site found at 358
    Illegal NgoMIV site found at 958
    Illegal NgoMIV site found at 1270
    Illegal NgoMIV site found at 1549
    Illegal NgoMIV site found at 2201
    Illegal NgoMIV site found at 2223
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4067


Design Notes

None


Source

E. coli & R. eutropha Tokyo Tech

References